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KMID : 0613819990090020160
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Journal of Life Science
1999 Volume.9 No. 2 p.160 ~ p.168
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Effects of Sodium Butyrate on the Biosynthesis of Sphingolipids in HT29, a Human Colon Cancer Cell Line
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Abstract
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Butyrate is one of the short-chain fatty acids that are present in the colon of mammals in millimolar concentration as a result of microbial anaerobic fermentation of dietary fiber, undigested starch, and proteins. In this study, sodium butyrate was examined in HT29 cell, Human colonic cancer cell line, on cell viability, alkaline phosphatase activity, PLC-$\gamma$ 1 expression and complex sphingolipid biosynthesis. Treatment with butyrate showed that the decrease of cell adhhesion and viability was time-dependent. Sodium butyrate also induced to increase the activity of alkaline phosphatase which is a differentiation marker enzyme and decreased so fast but ceramide was Biosythesis of sphingomyeline and galactosylceramide by butyrate treatment were decreased so fast but ceramide was increased 680dpm/mg protein% more than untreated froup on first day and then decrease fast. In addition, acid ceramidase and neutral ceramidase activity were inhibited early stage by sodium butyrate. These results suggest that sodium butyrate causes cell differentiation or cell growth arrest of HT29 cell accompained by early increase of ceramide content and alkaline phosphatase activity and decrease of galactosylceramide content and PLC-$\gamma$1 expression.
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KEYWORD
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